Adhesion and Nanomechanical Studies by Interfacial Force Microscopy

Cell adhesion force microscopy.

The adhesion forces of cervical carcinoma cells in tissue culture were measured by using the manipulation force microscope, a novel atomic force microscope. The forces were studied as a function of time and temperature for cells cultured on hydrophilic and hydrophobic polystyrene substrates with preadsorbed proteins. The cells attached faster and stronger at 37 degreesC than at 23 degreesC and ...

Bacterial adhesion force quantification by fluidic force microscopy.

Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physio...

Measuring interfacial and adhesion forces between bacteria and mineral surfaces with biological force microscopy

Interfacial and adhesion forces between living, unmodified bacterial cells (Escherichia coli) and mineral surfaces (muscovite, goethite, and graphite) have been directly measured in aqueous solution using a force microscope. Native cells are linked to a force-sensing probe that is used to characterize interactions as a mineral surface approaches, makes contact with, and withdraws from bacteria ...

Chemical Force Microscopy: Probing Chemical Origin of Interfacial Forces and Adhesion

Cantilever-Based Optical Interfacial Force Microscopy